About this role
Summary A Research Assistant I position will conduct research project focusing on immune cell-based cancer immunotherapy under supervision of Dr. Norihiro Watanabe.
The person will assist and perform various preclinical works including both in vitro (cell culture, molecular biology, flow cytometry, etc) and in vivo (handling mouse, imaging, bleeding, injection) experiments. Job Duties Assist lab member to conduct preclinical work under the guidance of PI as indicated below Perform aseptic cell culture including primary cells and cell lines Generate gene-modified therapeutic cells for preclinical work Conduct various in vitro functional assay such as cell surface phenotype (multi-color flow cytometry), Cytotoxicity assay (51Cr-release assay, Flow-based assay), cytokine detection (ELISA, Luminex assay), gene expression (Real-time PCR, RNA sequencing), and protein expression (flow cytometry, western blot) Generate virus constructs to enhance functionality of therapeutic immune cells (cloning, DNA/RNA isolation) Perform in vivo functional assay (mouse handling, imaging) Collect and analyze data Perform other job related duties as assigned Minimum Qualifications Bachelor's degree in a Basic Science or a related field.
Two years of relevant experience.
Preferred Qualifications
Bachelor's or Master's degree in a basic science, preferably Immunology, Cell Biology or Molecular Biology. Two years of laboratory work in industry or academia in a related field. Prior hands-on experience with cell culture, DNA/RNA processing, molecular cloning, gene transduction, flow cytometry, and handling mice.
Work Authorization Requirement: This position is not eligible for visa sponsorship. Candidates must be legally authorized to work in the United States at the time of application and throughout the duration of employment. Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.