Through its research in the fields of analytics, diagnostics, biosystems engineering and biosafety, the Bioanalytics and Bioprocesses Division (IZI-BB) addresses future-oriented and sustainable areas of research. The institute develops analytical and biotechnological solutions for medical issues, animal health, nutrition, cosmetics and the environment. In the Bioprocesses group we are looking for a Master’s student to complete their thesis. For cell-free protein synthesis systems to be used efficiently and reproducibly, targeted quality control of the underlying cell extracts is essential. In particular, the content of functional ribosomes and signal recognition particle (SRP) strongly determines the performance of eukaryotic cell-free protein synthesis systems. Within the scope of the Master’s thesis, quantitative assays will be developed and established to compare ribosome and SRP levels in eukaryotic lysates and to integrate these into a routine quality control protocol. Be part of change Planned work packages include: <ul> <li>Establishment and optimization of RNA isolation and RT qPCR protocols for quantitative detection of rRNA and 7SL RNA (SRP)</li> <li>Complementary hybridization-based methods (e.g. dot blot, simple FISH approaches) to validate RT qPCR results</li> <li>Biochemical characterization of lysates and correlation of the determined quality parameters with performance in cell-free protein synthesis reactions using complex model proteins</li> </ul> What you contribute We are looking for highly motivated and committed students who enjoy working in a creative and open research environment. We welcome the following qualifications and experience: <ul> <li>Ongoing Master’s studies in Biotechnology, Biology, Biochemistry or a related field</li> <li>Initial experience in molecular biology and biochemistry (e.g. PCR, plasmid preparation, protein detection methods); experience with RT qPCR and/or RNA work is an advantage but not mandatory</li> <li>Ability to work independently, careful and reliable working style, as well as team spirit and interest in interdisciplinary collaboration</li> </ul> What we offer <ul> <li>Opportunities to contribute to innovative research projects and put the knowledge you’ve gained during your studies into practice</li> <li>Access to state-of-the-art technology and laboratory facilities</li> <li>Supervision of your master’s thesis by researchers and feedback on an individual basis</li> </ul> We value and promote the diversity of our employees' skills and therefore welcome all applications – regardless of age, gender, nationality, ethnic and social origin, religion, ideology, disability, sexual orientation and identity. Severely disabled persons are given preference in the event of equal suitability. Our tasks are diverse and adaptable – for applicants with disabilities, we work together to find solutions that best promote their abilities. With its focus on developing key technologies that are vital for the future and enabling the commercial utilization of this work by business and industry, Fraunhofer plays a central role in the innovation process. As a pioneer and catalyst for groundbreaking developments and scientific excellence, Fraunhofer helps shape society now and in the future. Ready for a change? Then apply now and make a difference! Once we have received your online application, you will receive an automatic confirmation of receipt. We will then get back to you as soon as possible and let you know what happens next. If you have any further questions, feel free to contact: Dr. Anne Zemella Fraunhofer-Institute for Celltherapy und Immunology, Branch Bioanalytics und Bioprocesses, IZI-BB Am Mühlenberg 13, 14476 Potsdam, Germany +49 331 58187-332 [email protected] Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses <a href="http://www.izi-bb.fraunhofer.de" target="_blank" rel="noopener">www.izi-bb.fraunhofer.de</a> Requisition Number: 84753 Application Deadline:

Master's thesis in the field of cell-free synthesis systems (Potsdam, DE, 14476) @ Fraunhofer-Gesellschaft

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